Antiviral potential and stability analysis of chicken interferon-? produced by Newcastle disease virus in chicken embryo fibroblast cells
نویسندگان
چکیده
Antiviral potential and stability analysis of chicken interferon-? produced by Newcastle disease virus in embryo fibroblast cells | FR Anjum, SU Rahman, MA Aslam, AS Qureshi Agricultural Journals
منابع مشابه
Plaque formation of LaSota pathogenic strain of Newcastle disease virus adapted in chick embryo fibroblast cells
In order to adapt LaSota strain of Newcastle disease virus (NDV) on chick embryo fibroblast (CEF) cells, 0.1 ml from LaSota vaccine produced in Razi vaccine and serum research institute inoculated to the CEF cells and was passaged five times in the CEF grown in Eagle’s minimum essential medium (MEM). First to third passages were blind but in fourth passage cytopathic effect of virus was observe...
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RT-PCR for the detection of Newcastle disease virus (NDV) in allantoic fluids of SPF embryonated eggs as well as in tissues of SPF chickens infected experimentally is described. The method proved to be specific as all tested NDVs were detected and no cross reaction with other RNA viruses was observed. Sensitivity of the method was established at 10ELD50/0.1 ml. To detect NDV in chicken tissues,...
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Characteristic clinical manifestations of Newcastle disease include leukopenia and immunosuppression. Peripheral blood mononuclear cells (PBMCs) are the main targets of Newcastle disease virus (NDV) infection. To survey changes in proteomic expression in chicken PBMCs following NDV infection, PBMC proteins from 30 chickens were separated using two- dimensional electrophoresis (2-DE) and subject...
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Background: The specific antibody response against Newcastle disease virus (NDV) in the blood serum of chickens hatched from eggs exposed to low dose gamma-radiation was studied. Materials and methods: Two groups of eggs of commercial meat chicken lines were irradiated with the dose of 0.30 Gy 60Co gamma-rays before incubation and on the 19th day of incubation, respectively. The same num...
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Marek’s disease (MD), a lymphoproliferative disorder of chickens caused by the MD virus (MDV), is economically significant. The resistance/susceptibility to MD is controlled by host genetics. The host response to different virus strains varies. The pathogenicity of REV-LTR deleted GX0101∆LTR MDV has been previously reported. However, the precise molecular mechanism of the response of chickens t...
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ژورنال
عنوان ژورنال: Veterinarni Medicina
سال: 2021
ISSN: ['1805-9392', '0375-8427']
DOI: https://doi.org/10.17221/106/2020-vetmed